High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis
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Authors
Laremore, Tatiana N.
Ly, Mellisa
Solakyildirim, Kemal
Zagorevski, Dmitri V.
Linhardt, Robert J.
Issue Date
2010-01-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
Description
Analytical Biochemistry, 401, 236–241
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis, T. N. Laremore, M. Ly, K. Solakyildirim, D.V. Zagorevski, R. J. Linhardt, Analytical Biochemistry, 401, 236–241, 2010.
Publisher
Elsevier
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697