Structural characterization and interaction with RCA120 of a highly sulfated keratan sulfate from blue shark (Prionace glauca) cartilage
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Authors
Li, Q.
Li, G.
Zhao, X.
Shan, X.
Cai, C.
Zhao, J.
Zhang, F.
Linhardt, Robert J.
Yu, G.
Issue Date
2018
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
As an important glycosaminoglycan, keratan sulfate (KS) mainly exists in corneal and cartilage, possessing various biological activities. In this study, we purified KS from blue shark (Prionace glauca) cartilage and prepared KS oligosaccharides (KSO) through keratanase II-catalyzed hydrolysis. The structures of KS and KSO were characterized using multi-dimensional nuclear magnetic resonance (NMR) spectra and liquid chromatography-mass spectrometry (LC-MS). Shark cartilage KS was highly sulfated and modified with ~2.69% N-acetylneuraminic acid (NeuAc) through α(2,3)-linked to galactose. Additionally, KS exhibited binding affinity to Ricinus communis agglutinin I (RCA120) in a concentration-dependent manner, a highly toxic lectin from beans of the castor plant. Furthermore, KSO from dp2 to dp8 bound to RCA120 in the increasing trend while the binding affinity of dp8 was superior to polysaccharide. These results define novel structural features for KS from Prionace glauca cartilage and demonstrate the potential application on ricin-antidote exploitation.
Description
Marine Drugs, 16, 128
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Structural characterization and interaction with RCA120 of a highly sulfated keratan sulfate from blue shark (Prionace glauca) cartilage, Q. Li, G. Li, X.Zhao, X. Shan, C. Cai, J. Zhao, F. Zhang, R. J. Linhardt, G. Yu, Marine Drugs, 16, 128, 2018.
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)