Suppression of Tumor Growth by a New Glycosaminoglycan Isolated from African Giant Snail Achatina fulica
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Authors
Lee, Yeon Sil
Yang, Hyun Ok
Shin, Kuk Hyun
Choi, Hyung Seok
Jung, Sang Hoon
Kim, Yong Man
Oh, Deok Kun
Linhardt, Robert J.
Kim, Yeong Shik
Issue Date
2003-03-28
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Acharan sulfate is a new type of glycosaminoglycan from the giant African snail, Achatina fulica. Acharan sulfate, which has a primary repeating disaccharide structure of alpha-D-N-acetylglucosaminyl-2-O-sulfo-alpha-L-iduronic acid, was studied as a potential antitumor agent in both in vivo and in vitro assays. The antiangiogenic activity of acharan sulfate was evaluated in the chorioallantoic membrane assay and by measuring its effect on the proliferation of calf pulmonary artery endothelial cells. In vivo, a matrigel plug assay showed that acharan sulfate suppressed basic fibroblast growth factor (bFGF)-stimulated angiogenesis and lowered the hemoglobin (Hb) content inside the plug. Acharan sulfate was administered s.c. at two doses for 15 days to C57BL/6 mice implanted with murine Lewis lung carcinoma in the back. It was also administered i.p. to ICR mice bearing sarcoma 180 at a dose of 30 mg/kg. Subcutaneous injection of acharan sulfate at doses of 10 and 30 mg/kg decreased tumor weight and tumor volume by 40% without toxicity or resistance. Intraperitoneal injection of acharan sulfate also decreased tumor weight and volume by 40% in sarcoma 180-bearing mice. These results suggest that the antitumor activity of acharan sulfate may be related to the inhibition of angiogenesis.
Description
European Journal of Pharmocology, 465, 191-198
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Suppression of Tumor Growth by a New Glycosaminoglycan Isolated from African Giant Snail Achatina fulica, Y.S. Lee, K.H. Shin, D.K. Oh, R.J. Linhardt, Y.S. Kim, European Journal of Pharmocology, 465, 191-198, 2003.
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ISSN
142999