Chemometric analysis of porcine, bovine and ovine heparins

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Authors
Ouyang, Yilan
Han, Xiaorui
Yu, Yanlei
Chen, Jianle
Fu, Li
Zhang, Fuming
Linhardt, Robert J.
Fareed, Jawed
Hoppensteadt, Debra
Jeske, Walter
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Issue Date
2019-02-05
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Heparin is a polysaccharide anticoagulant drug isolated from animal tissues. There have been concerns on the safety and security of the heparin supply chain since 2007-8 when a contamination crisis led to its disruption. The current study applies a suite of modern analytical techniques to porcine, bovine and ovine intestinal mucosal heparins. These techniques include structural analysis by nuclear magnetic resonance spectrometry, disaccharide compositional analysis, bottom-up analysis of tetrasaccharides corresponding to heparin's antithrombin III binding site. Chemometric analysis was then applied to understand how these structural differences to predict the animal/tissue source of heparin and to help detect blending of heparins from various sources.
Description
Journal of Pharmaceutical and Biomedical Analysis, 164, 345–352
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Full Citation
Chemometric analysis of porcine, bovine and ovine heparins, Y. Ouyang, X. Han, Y. Yu, J. Chen, F. Zhang, R. J. Linhardt, J. Fareed, D. Hoppensteadt, W. Jeske, A. Kouta, Z. Zhang, K. Xia, Journal of Pharmaceutical and Biomedical Analysis, 164, 345–352, 2019.
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https://doi.org/10.1016/j.jpba.2018.10.052
 https://hdl.handle.net/20.500.13015/5574
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ISSN
1873264X
7317085
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Linhardt Research Labs Papers