Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide

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Authors
Huang, Haichan
Liu, Xiaobo
Lv, Shencong
Zhong, Weihong
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2016-09-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
Heparosan, the capsular polysaccharide of Escherichia coli K5 having a carbohydrate backbone similar to that of heparin, has become a potential precursor for bioengineering heparin. In the heparosan biosynthesis pathway, the gene waaR encoding α-1-, 2- glycosyltransferase catalyze s the third glucosyl residues linking to the oligosaccharide chain. In the present study, a waaR deletion mutant of E. coli K5 was constructed. The mutant showed improvement of capsule polysaccharide yield. It is interesting that the heparosan molecular weight of the mutant is reduced and may become more suitable as a precursor for the production of low molecular weight heparin derived from the wild-type K5 capsular polysaccharide.
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Applied Microbiology and Biotechnology, 100, 7877–7885
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Full Citation
Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide, H. Huang, X. Liu, S. Lv, W. Zhong, F. Zhang, R.J. Linhardt, Applied Microbiology and Biotechnology, 100, 7877–7885, 2016
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ISSN
14320614
1757598
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