Chemoenzymatic Synthesis of Low Molecular Weight Heparin and Heparan Sulfate

Authors
Zhang, X.
Linhardt, Robert J.
ORCID
https://orcid.org/0000-0003-2219-5833
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Other Contributors
Issue Date
2017
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Degree
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Full Citation
Chemoenzymatic Synthesis of Low Molecular Weight Heparin and Heparan Sulfate, X. Zhang, R. J. Linhardt, Chapter 8, pp 233-252, in Chemical Biology of Glycoproteins: A tool for understanding and engineering glycans attached to proteins, Z. Tan and L.-X.Wang (Eds.), Royal Society of Chemistry, Cambridge, UK, 2017.
Abstract
Heparan sulfate is a polysaccharide that plays essential physiological functions in the animal kingdom. Heparin, a highly sulfated form of heparan sulfate, is a widely prescribed anticoagulant drug worldwide. The heparan sulfate and heparin isolated from natural sources are highly heterogeneous mixtures differing in polysaccharide chain lengths and sulfation patterns. The access to structurally defined heparan sulfate and heparin is critically important to probe the contribution of specific sulfated saccharide structures to biological functions as well as for the development of the next generation of heparin-based anticoagulant drugs. The synthesis of heparan sulfate and heparin, using a purely chemical approach, has proven extremely difficult, especially for targets larger than octasaccharides having a high degree of site-specific sulfation. A new chemoenzymatic method has emerged as an effective alternative approach. This method utilizes recombinant heparan sulfate biosynthetic enzymes combined with unnatural uridine diphosphate-monosaccharide donors. Recent examples demonstrate the successful synthesis of ultra-low molecular weight heparin, low-molecular weight heparin and bioengineered heparin with unprecedented efficiency. The new method opens the opportunity to develop improved heparin-based therapeutics.
Description
Chapter 8, pp 233-252, in Chemical Biology of Glycoproteins: A tool for understanding and engineering glycans attached to proteins, Z. Tan and L. X.Wang (Eds.), Royal Society of Chemistry, Cambridge, UK
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
https://harc.rpi.edu/
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