Investigations into multimodal chromatographic selectivity using in silico designed Fab fragment variants and column modeling
Authors
Karkov, Hanne Sophie
Issue Date
2015-05
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemical and biological engineering
Alternative Title
Abstract
The Fab variants were employed to investigate the effects of changing the mobile phase conditions and multimodal ligand design. Using arginine as a mobile phase modifier, Fab variant elution was promoted at a lower ionic strength. In contrast, the presence of guanidine amplified the selective trends of the multimodal system and created a window of selectivity for the separation of Fab variants which could not be separated in the system without the modifier. A novel set of multimodal chromatographic ligands were employed to examine the effects of changing the ligand structure on Fab variant retention and selectivity. Interestingly, the multimodal chromatographic ligands exhibited different selectivity trends and were capable of differentiating between different Fab classes and related variants.
Description
May 2015
School of Engineering
School of Engineering
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY