NMR studies of the chiral and chemical interactions of self-assembled guanosine monophosphate
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Authors
Gupta, Akshar P.
Issue Date
2013-12
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemistry
Alternative Title
Abstract
This dissertation explores the chiral selectivity exhibited by GMP assemblies, and their interactions in nucleotide mixtures. Previous work in the McGown group demonstrated that GMP can separate enantiomers using capillary electrophoresis (CE). However, the quality of chiral selection was dependent on the extended structure of each solute, for example, easily resolving enantiomers of tryptophan, but not amino acids with analogous, but truncated aromatic side chains (phenylalanine and tyrosine). Thus, investigated here are the structural features of GMP assemblies and their interactions with chiral compounds to reveal the basis for enantioselectivity. Solution-state 1H nuclear magnetic resonance (NMR) was used to map site-specific changes in the chemical shifts within both GMP and the chiral solutes.
Description
December 2013
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY