Novel mechanistic insights into the pressure and temperature stabilization of enzymes
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Authors
Vasilchuk, Daniel
Issue Date
2016-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemistry
Alternative Title
Abstract
Pressure perturbation calorimetry (PPC) was used to determine the volumetric properties for a diverse set of globular proteins, including six ancestral thioredoxins (Trx). The thermal expansion coefficient in the native state as a function of temperature (αN (T)) was shown to be very different for the studied proteins. After eliminating various structural and thermodynamic factors it was concluded that the differences in the native state expansivity function might be defined by the overall tertiary topology of the protein fold; a hypothesis that was supported by the data for the ancestral Trx. Also, the αN values were slightly different even for proteins with the same topology (Trx).
Description
August 2016
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY