Comparison of low-molecular-weight heparins prepared from ovine heparins with enoxaparin

Authors
Chen, Jianle
Yu, Yanlei
Fareed, Jawed
Hoppensteadt, Debra
Jeske, Walter
Kouta, Ahmed
Jin, Caijuan
Jin, Yongsheng
Yao, Yiming
Xia, Ke
ORCID
https://orcid.org/0000-0003-2219-5833
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Issue Date
2019-04-12
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Terms of Use
Attribution-NonCommercial 3.0 United States
CC BY-NC : This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. . The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
Full Citation
Comparison of low-molecular-weight heparins prepared from ovine heparins with enoxaparin, J. Chen, Y. Yu, J. Fareed, D. Hoppensteadt, W. Jeske, A. Kouta, C. Jin, Y. Jin, Y. Yao, K. Xia, F. Zhang, S. Chen, X. Ye, R. J. Linhardt, Clinical and Applied Thrombosis/Hemostasis, 25, 1-9, 2019.
Abstract
Heparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants. These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism. Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung. The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues. A number of laboratories are now also examining the similarities between heparin and low-molecular-weight heparins prepared from porcine and ovine tissues. This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin. Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin. Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared. Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox. Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin. The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.
Description
Clinical and Applied Thrombosis/Hemostasis, 25, 1-9
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Department
The Linhardt Research Labs.
The Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
Publisher
Sage
Relationships
The Linhardt Research Labs Online Collection
Rensselaer Polytechnic Institute, Troy, NY
Clinical and Applied Thrombosis/Hemostasis
https://harc.rpi.edu/
Access
Open Access
CC BY-NC — Creative Commons Attribution