Comparison of low-molecular-weight heparins prepared from ovine heparins with enoxaparin

dc.contributor.authorChen, Jianle
dc.contributor.authorYu, Yanlei
dc.contributor.authorFareed, Jawed
dc.contributor.authorHoppensteadt, Debra
dc.contributor.authorJeske, Walter
dc.contributor.authorKouta, Ahmed
dc.contributor.authorJin, Caijuan
dc.contributor.authorJin, Yongsheng
dc.contributor.authorYao, Yiming
dc.contributor.authorXia, Ke
dc.contributor.authorZhang, Fuming
dc.contributor.authorChen, Shiguo
dc.contributor.authorYe, Xingqian
dc.contributor.authorLinhardt, Robert J.
dc.descriptionClinical and Applied Thrombosis/Hemostasis, 25, 1-9
dc.descriptionNote : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
dc.description.abstractHeparin and its low-molecular-weight heparin derivatives are widely used clinical anticoagulants. These drugs are critical for the practice of medicine in applications, including kidney dialysis, cardiopulmonary bypass, and in the management of venous thromboembolism. Currently, these drugs are derived from livestock, primarily porcine intestine and less frequently bovine intestine and bovine lung. The worldwide dependence on the pig as a single dominant animal species has made the supply chain for this critical drug quite fragile, leading to the search for other sources of these drugs, including the expanded use of bovine tissues. A number of laboratories are now also examining the similarities between heparin and low-molecular-weight heparins prepared from porcine and ovine tissues. This study was designed to compare low-molecular-weight heparin prepared from ovine heparin through chemical β-elimination, a process currently used to prepare the low-molecular-weight heparin, enoxaparin. Using top-down, bottom-up, and compositional analyses as well as bioassays, low-molecular-weight heparin derived from ovine intestine was shown to closely resemble enoxaparin. Moreover, the compositions of daughter low-molecular-weight heparins prepared from three unfractionated ovine parent heparins were compared. Ovine enoxaparins had similar molecular weight and in vitro anticoagulant activities as Lovenox. Some disaccharide compositional, oligosaccharide composition at the reducing and nonreducing ends and intact chain compositional differences could be observed between porcine enoxaparin and ovine low-molecular-weight heparin. The similarity of these ovine and porcine heparin products suggests that their preclinical evaluation and ultimately clinical assessment is warranted.
dc.description.sponsorshipNational Institutes of Health
dc.identifier.citationComparison of low-molecular-weight heparins prepared from ovine heparins with enoxaparin, J. Chen, Y. Yu, J. Fareed, D. Hoppensteadt, W. Jeske, A. Kouta, C. Jin, Y. Jin, Y. Yao, K. Xia, F. Zhang, S. Chen, X. Ye, R. J. Linhardt, Clinical and Applied Thrombosis/Hemostasis, 25, 1-9, 2019.
dc.relation.departmentThe Linhardt Research Labs.
dc.relation.departmentThe Shirley Ann Jackson, Ph.D. Center for Biotechnology and Interdisciplinary Studies (CBIS)
dc.relation.ispartofThe Linhardt Research Labs Online Collection
dc.relation.ispartofRensselaer Polytechnic Institute, Troy, NY
dc.relation.ispartofClinical and Applied Thrombosis/Hemostasis
dc.rightsAttribution-NonCommercial 3.0 United States*
dc.rights.holderCC BY-NC : This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator. . The license allows for commercial use. Credit must be given to the authors and the original work must be properly cited.
dc.rights.licenseCC BY-NC — Creative Commons Attribution
dc.subjectChemistry and chemical biology
dc.subjectChemical and biological engineering
dc.subjectBiomedical engineering
dc.titleComparison of low-molecular-weight heparins prepared from ovine heparins with enoxaparinen_US
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