A combined experimental approach to investigate the underlying mechanisms of history-dependent phenomena in skeletal muscle : the Drosophila jump muscle and single fiber tissue engineering

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Authors
Koppes, Ryan Alan
Issue Date
2013-05
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Electronic thesis
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ENG
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Biomedical engineering
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Abstract
Alternatively, muscle fibers engineered from mammalian skeletal muscle progenitor cells may provide an additional experimental platform to investigate history-dependent phenomena. Muscle fibers produced in vitro would allow for genetic modification capable in mammalian culture systems without the need for animal use or complicated dissections. Additionally, the engineering of functional muscle tissue in vitro may provide a successful strategy to address the clinical demand for muscle replacement. The loss of skeletal muscle function due to genetic or acquired conditions, such as traumatic injury, disease, or surgical excisions, causes a physiological deficit that remains without an effective clinical treatment. Current attempts to engineer muscle in vitro has been limited to two-dimensions or relied on scaffold-based approaches and have yet to achieve the dynamic mechanical responses of physiological muscle. To this end, we have developed a self-assembling, scaffold-free approach in conjunction with an electromechanical bioreactor to facilitate the engineering of three-dimensional functional muscle fibers. In addition to providing a platform to study muscle mechanics, including history-dependent behaviors, muscle fibers grown in vitro will offer a unique model to study muscle development, specifically the cues important for differentiating slow and fast muscle fibers.
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May 2013
School of Engineering
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Rensselaer Polytechnic Institute, Troy, NY
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