Quantitation of heparosan with heparin lyase III and spectrophotometry
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Authors
Huang, Haichan
Zhao, Yingying
Lv, Shencong
Zhong, Weihong
Zhang, Fuming
Linhardt, Robert J.
Issue Date
2014-02-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Heparosan is Escherichia coli K5 capsule polysaccharide, which is the key precursor for preparing bioengineered heparin. A rapid and effective quantitative method for detecting heparosan is important in the large-scale production of heparosan. Heparin lyase III (Hep III) effectively catalyzes the heparosan depolymerization, forming unsaturated disaccharides that are measurable using a spectrophotometer at 232 nm. We report a new method for the quantitative detection of heparosan with heparin lyase III and spectrophotometry that is safer and more specific than the traditional carbazole assay. In an optimized detection system, heparosan at a minimum concentration of 0.60 g/L in fermentation broth can be detected.
Description
Analytical Biochemistry, 447, 46–48
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Quantitation of heparosan with heparin lyase III and spectrophotometry, H. Huang, Y. Zhao, S. Lv, W. Zhong, F. Zhang，R. J. Linhardt, Analytical Biochemistry, 447, 46–48, 2014
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697