Molecular and biochemical profiling of a heparin-derived oligosaccharide, C3

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Authors
Ma, Qing
Dudas, Bertalan
Daud, Asif
Iqbal, Omer
Hoppensteadt, Debra
Jeske, Walter
Cornelli, Umberto
Lee, John
Lorens, Stanley
Mervis, Ronald
Issue Date
2002-02-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
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Abstract
This study was designed to characterize a heparin-derived oligosaccharide (HDO), C3, using chemical and biochemical methods. Although previous studies have suggested C3 as a promising compound in the treatment of Alzheimer's disease (AD), its molecular and biochemical properties are still unknown. In this study, the molecular profiles and anticoagulant effects of C3 were investigated. To characterize the molecular and biochemical properties of C3, gel permeation chromatography (GPC), polyacrylmide gel electrophoresis (PAGE), radiolabeling and anticoagulant assays, such as activated partial thromboplastin time (APTT), Heptest, and anti-factor Xa assay, were used. The GPC profile revealed that C3 was an ultra-low-molecular-weight (MW) heparin mixture. The multiple components in C3 were studied with PAGE analysis. Tritium-labeled C3 exhibited similar biological properties as nonlabeled materials. The biological assays showed that C3 and its components exhibited weak anticoagulant effect. These results demonstrated the applicability of the combination of GPC, PAGE, and coagulation assays to characterize the molecular and biochemical profile of HDO. In addition, the low anticoagulant effect of C3 suggests that this compound could be a relatively low-risk adjunct in the treatment of AD.
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Thrombosis Research, 105, 303-309
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Full Citation
Molecular and biochemical profiling of a heparin-derived oligosaccharide, C3 Q. Ma, B. Dudas, A. Daud, O. Iqbal, D. Hoppensteadt, W. Jeske, U. Cornelli, J. Lee, S. Lorens, R. Mervis, I. Hanin, I. Capila, R. Linhardt, J. Fareed, Thrombosis Research, 105, 303-309, 2002.
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493848
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