A Colorimetric Assay for Chemical Heparin in Plasma
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Authors
Klein, Michael D.
Drongowski, Robert A.
Linhardt, Robert J.
Langer, Robert S.
Issue Date
1982-07-15
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
A simple, rapid colorimetric assay for plasma heparin is presented. The assay employs the metachromasia of azure A when heparin is added. It is useful for 0 to 10 units/ml and does not depend on heparin's anticoagulant activity. Heparin concentrations determined with this assay are not exactly the same as those determined with coagulation assays. This is probably because azure A determines chemical heparin, not anticoagulant active heparin.
Description
Analytical Biochemistry, 124, 59-64
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
A Colorimetric Assay for Chemical Heparin in Plasma, M. Klein, R.A. Drongowski, R.J. Linhardt, R. Langer, Analytical Biochemistry, 124, 59-64 (1982).
Publisher
Terms of Use
Journal
Volume
Issue
PubMed ID
DOI
ISSN
10960309
32697
32697