Investigations of the aptamer capability of g-quadruplex-forming oligonucleotides
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Authors
Albanese, Christina M.
Issue Date
2016-08
Type
Electronic thesis
Thesis
Thesis
Language
ENG
Keywords
Chemistry
Alternative Title
Abstract
The second focus of this dissertation explores the selectivity and diagnostic potential of the G-quadruplex-forming VEGF aptamer. This aptamer selectively binds the VEGF protein, which is present at high serum levels in numerous cancers and diseases. Using an affinity MALDI MS platform, we covalently attached the VEGF aptamer to fused silica MALDI probe surfaces to selectively capture the VEGF protein from a complex mixture of human serum proteins. These results demonstrate the value of our affinity MALDI method and may allow for rapid protein screening of diseases characterized by high VEGF serum levels.
Description
August 2016
School of Science
School of Science
Full Citation
Publisher
Rensselaer Polytechnic Institute, Troy, NY