Anti-inflammation effects of highly purified low-Mw RG-I pectins on LPS-activated macrophages
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Authors
Wu, Dongmei
Zheng, Xiaoliang
Hu, Weiwei
Zhu, Kai
Yu, Chengxiao
He, Qiaojun
Linhardt, Robert J.
Ye, Xingqian
Chen, Shiguo
Issue Date
2021-11-01
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Pectic oligosaccharides are viewed as excellent immunomodulator, but the specific molecular weight (Mw) range required for anti-inflammatory effects remains unclear. In this study, citrus pectins was depolymerized by the ultrasound/H2O2/Vc system and the pectic mixtures was separated through the superdex 30 chromatography column. Three highly purified pectin fractions (USP1, USP2, USP3) were obtained, with the Mw of 2713 g/mol, 3683 g/mol, and 7469 g/mol. All of them were RG-I pectins and USP3 contained more arabinan sidechains than USP1 and USP2. In vitro anti-inflammation assays based on LPS-induced RAW264.7 macrophages indicated that these pectins could suppress the NO release, ROS production and down-regulated the level of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in a Mw-dependent manner. The inhibitory effects increased in the order of USP1<USP2<USP3. Meanwhile, the regulation of pro-inflammatory cytokines were closely related to the content of arabinan sidechains in pectin. Thus, low-Mw pectins with Mw>3KD should be effective as a prophylactic agent against inflammatory diseases in functional food.
Description
Bioactive Carbohydrates and Dietary Fibre, 26, 100283
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Anti-inflammation effects of highly purified low-Mw RG-I pectins on LPS-activated macrophages, D. Wu, X. Zheng, W. Hu, K. Zhu, C. Yu, Q. He, R. J. Linhardt, X. Ye, S. Chen, Bioactive Carbohydrates and Dietary Fibre, 26, 100283, 2021.
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DOI
ISSN
22126198