Controlled Release of Protein and Vaccines from Poly(Ester) Microspheres In Vitro
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Authors
Wang, H.T.
Palmer, H.
Linhardt, Robert J.
Flanagan, D.R.
Schmitt, E.
Issue Date
1991
Type
Article
Language
ENG
Keywords
Biology , Chemistry and chemical biology , Chemical and biological engineering , Biomedical engineering
Alternative Title
Abstract
Biodegradable microspheres of poly(L-lactide) and copolymers of lactide and glycolide have been prepared by spray drying. Degradation studies of these microspheres using residual mass measurements, viscometry and gel permeation chromatography indicated the entire mass of polyester matrices was maintained for 10, 30 days and 6–10 months for 50:50 and 85:15 copolymers and poly(L-lactide), respectively. The continuous drop in polymer intrinsic viscosity and molecular weight during hydrolysis suggested that matrix degradation began as soon as these microspheres were placed in the buffer and that their degradation proceeded through random-chain scission. Protein release, using bovine serum albumin micro-spheres, showed that release from 50:50 copolymer was independent of polymer molecular weight over a range from 31,000 to 93,000. The release was, however, dependent on the polymer composition and BSA loading in the microspheres. A burst-effect was found in the release study for microspheres prepared from copolymers. The identity and integrity of the released protein was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis on the release products of polymeric microspheres containing BSA, FPL-R and EcoBacR-Plus vaccines. These results suggested that the release BSA (or vaccine) from polymeric microspheres could be sustained for up to one month.
Description
in Cosmetic and Pharmaceutical Applications of Polymers, Plenum Publishing Company, New York, C.G. Gebelein, T.C. Cheng, V.C. Yang (Eds.), pp 239-253
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Note : if this item contains full text it may be a preprint, author manuscript, or a Gold OA copy that permits redistribution with a license such as CC BY. The final version is available through the publisher’s platform.
Full Citation
Controlled Release of Protein and Vaccines from Poly(Ester) Microspheres In Vitro, H.T. Wang, H. Palmer, R.J. Linhardt, D.R. Flanagan, E. Schmitt, in Cosmetic and Pharmaceutical Applications of Polymers, Plenum Publishing Company, New York, C.G. Gebelein, T.C. Cheng, V.C. Yang (Eds.), pp 239-253, 1991.